Biomonitoring

We offer a broad range of LC-MS and GC-MS based methods for the quantitative determination of environmental chemicals and/or their specific metabolites in various biological matrices, such as urine, serum, plasma or saliva. Our methods are frequently used on biomonitoring campaigns to evaluate the (non-)occupational exposure to environmental chemicals.

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Biomarker	Exposure to	Matrix	Analytical Technique	Reference
1-Hydroxyphenanthrene (1-OH-Phe)	Phenanthrene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
1-Hydroxypyrene (1-OH-Pyr)	Pyrene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
1-Naphthol (1-Hydroxynaphthalene, 1-OH-Nap)	Naphthalene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
2,2,4-Trimethyl-6(2H)-quinolinone (EQI)	Ethoxyquin	Urine	LC-MS/MS
2-(4-amino-2-hydroxybenzoyl)benzoic acid (AHB)	Uvinul A plus	Urine	LC-MS/MS	publication March 2020 Human metabolism and urinary excretion kinetics of the UV filter Uvinul A plus® after a single oral or dermal dosage Stoeckelhuber M, Scherer M, Peschel O, Leibold E, Bracher F, Scherer G, Pluym N International Journal of Hygiene and Environmental Health, 227:113509, doi: 10.1016/j.ijheh.2020.113509 publication November 2019 A validated UPLC-MS/MS method for the determination of urinary metabolites of Uvinul® A plus Stoeckelhuber M, Pluym N, Bracher F, Leibold E, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, volume 411, doi: 10.1007/s00216-019-02201-6
2-(4-diethylamino-2-hydroxybenzoyl)benzoic acid (DHB)	Uvinul A plus	Urine	LC-MS/MS	publication March 2020 Human metabolism and urinary excretion kinetics of the UV filter Uvinul A plus® after a single oral or dermal dosage Stoeckelhuber M, Scherer M, Peschel O, Leibold E, Bracher F, Scherer G, Pluym N International Journal of Hygiene and Environmental Health, 227:113509, doi: 10.1016/j.ijheh.2020.113509 publication November 2019 A validated UPLC-MS/MS method for the determination of urinary metabolites of Uvinul® A plus Stoeckelhuber M, Pluym N, Bracher F, Leibold E, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, volume 411, doi: 10.1007/s00216-019-02201-6
2-(4-ethylamino-2-hydroxybenzoyl)benzoic acid (EHB)	Uvinul A plus	Urine	LC-MS/MS	publication March 2020 Human metabolism and urinary excretion kinetics of the UV filter Uvinul A plus® after a single oral or dermal dosage Stoeckelhuber M, Scherer M, Peschel O, Leibold E, Bracher F, Scherer G, Pluym N International Journal of Hygiene and Environmental Health, 227:113509, doi: 10.1016/j.ijheh.2020.113509 publication November 2019 A validated UPLC-MS/MS method for the determination of urinary metabolites of Uvinul® A plus Stoeckelhuber M, Pluym N, Bracher F, Leibold E, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, volume 411, doi: 10.1007/s00216-019-02201-6
2-Hydroxy-1-phenylethylmercapturic acid (PHEMA 1)	Styrene	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
2-Hydroxy-2-phenylethylmercapturic acid (PHEMA 2)	Styrene	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
2-Hydroxyethylmercapturic acid (HEMA)	Ethylene oxide, Alkylating agents	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients – methods Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
2-Hydroxyfluorene (2-OH-Flu)	Fluorene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon
2-Hydroxyphenanthrene (2-OH-Phe)	Phenanthrene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
2-Hydroxypropylmercapturic acid (2-HPMA)	Propylene oxide	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients – methods Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2018 Biomarkers of exposure specific to e-vapor products based on stable-isotope labeled ingredients Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N SRNT 2018, Baltimore, USA Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA publication June 2021 Assessment of the potential vaping-related exposure to carbonyls and epoxides using stable isotope-labeled precursors in the e-liquid Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Niessner R, Pluym N Archives of Toxicology, doi:10.1007/s00204-021-03097-x publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
2-Naphthol (2-Hydroxynaphthalene, 2-OH-Nap)	Naphthalene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
3-Hydroxyphenanthrene (3-OH-Phe)	Phenanthrene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
3-Hydroxypropylmercapturic acid (3-HPMA)	Acrolein	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients – methods Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2018 Biomarkers of exposure specific to e-vapor products based on stable-isotope labeled ingredients Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N SRNT 2018, Baltimore, USA Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA publication June 2021 Assessment of the potential vaping-related exposure to carbonyls and epoxides using stable isotope-labeled precursors in the e-liquid Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Niessner R, Pluym N Archives of Toxicology, doi:10.1007/s00204-021-03097-x publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
3-hydroxybenzo[a]pyrene (3-OH-BaP)	Benzo[a]pyrene	Urine	LC-MS/MS	Conference contribution June 2021 Analysis of 3-hydroxybenzo[a]pyrene in urine as a biomarker of exposure for benzo[a]pyrene in smokers and users of potentially reduced risk products Rögner N, Scherer M, Scherer G, Pluym N Global Forum on Nicotine 2021, Virtual publication October 2021 A Sensitive LC–MS/MS Method for the Quantification of 3-Hydroxybenzo[a]pyrene in Urine-Exposure Assessment in Smokers and Users of Potentially Reduced-Risk Products Rögner N, Hagedorn H-W, Scherer G, Scherer M, Pluym N Separations, 8(10), 171, doi: 10.3390/separations8100171 Conference contribution October 2021 Analysis of 3-hydroxybenzo[a]pyrene in urine as a biomarker of exposure for benzo[a]pyrene in smokers and users of potentially reduced risk products Scherer M, Rögner N, Scherer G, Pluym N CORESTA SSPT 2021, Virtual publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
4-Hydroxyphenanthrene (4-OH-Phe)	Phenanthrene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
7-Hydroxycitronellylic acid	7-Hydroxycitronellal	Urine	LC-MS/MS	publication March 2020 Biomonitoring data on young adults from the Environmental Specimen Bank suggest a decrease in the exposure to the fragrance chemical 7-hydroxycitronellal in Germany from 2000 to 2018 Pluym N, Petreanu W, Weber T, Scherer G, Scherer M, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, 227:113508, doi: 10.1016/j.ijheh.2020.113508 publication October 2017 Human metabolism and excretion kinetics of the fragrance 7-hydroxycitronellal after a single oral or dermal dosage Stoeckelhuber M, Krnac D, Pluym N, Scherer M, Peschel O, Leibold E, Scherer G International Journal of Hygiene and Environmental Health, Volume 221, doi: 10.1016/j.ijheh.2017.10.015 publication October 2017 A validated UPLC-MS/MS method for biomonitoring the exposure to the fragrance 7-hydroxycitronellal Stoeckelhuber M, Krnac D, Pluym N, Scherer M, Leibold E, Scherer G Journal of Chromatography B, Volumes 1068–1069, doi: doi.org/10.1016/j.jchromb.2017.10.040
8-Carboxygeraniol	Geraniol	Urine	LC-MS/MS	publication January 2022 Time trend of the exposure to geraniol in 24-h urine samples derived from the German Environmental Specimen Bank from 2004 to 2018 Pluym N, Stoeckelhuber M, Weber T, Scherer G, Scherer M, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 239, doi.org/10.1016/j.ijheh.2021.113880
9-Hydroxyphenanthrene (9-OH-Phe)	Phenanthrene	Urine	LC-MS/MS	publication February 2020 Polycyclic aromatic hydrocarbons (PAH) in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Roth A, Schwedler G, Schmied-Tobies MIH, Rucic E, Pluym N, Scherer M, Scherer G, Conrad A, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 226, doi: 10.1016/j.ijheh.2020.113491 publication November 2010 A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of phenolic polycyclic aromatic hydrocarbons (OH-PAH) in urine of non-smokers and smokers Ramsauer B, Sterz K, Hagedorn H-W, Engl J, Scherer G, McEwan M, Errington G, Shepperd J, Cheung F Analytical and Bioanalytical Chemistry, volume 399, doi:10.1007/s00216-010-4355-7 publication August 2022 Assessment of the exposure to polycyclic aromatic hydrocarbons in users of various tobacco/nicotine products by suitable urinary biomarkers Gerhard Scherer, Max Scherer, Nadine Rögner, Nikola Pluym Archives of Toxicology doi.org/10.1007/s00204-022-03349-4 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
Acrylamidemercapturic acid (AAMA)	Acrylamide	Urine	LC-MS/MS	publication October 2020 Benzene metabolite SPMA and acrylamide metabolites AAMA and GAMA in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Schwedler G, Murawski A, Schmied-Tobies MIH, Rucic E, Scherer M, Pluym N, Scherer G, Bethke R, Kolossa-Gehring M Environmental Research, Volume 192, doi: 10.1016/j.envres.2020.110295 publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x publication March 2006 Urinary Mercapturic Acids and a Hemoglobin Adduct for the Dosimetry of Acrylamide Exposure in Smokers and Nonsmokers Urban M, Kavvadias D, Riedel K, Scherer G, Tricker AR Inhalation Toxicology, Volume 18, doi: 10.1080/08958370600748430 Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Carboxyhemoglobin (COHb)	Carbon monoxide (CO)	Blood	GC-MS
Cotinine	Nicotine	Urine, Plasma, Serum, Saliva	LC-MS/MS	publication March 2019 Biomarkers of Exposure Specific to E-vapor Products Based on Stable-Isotope Labeled Ingredients Landmesser A, Scherer M, Pluym N, Sarkar M, Edmiston J, Niessner R, Scherer G Nicotine & Tobacco Research, Volume 21, doi: 10.1093/ntr/nty204 publication May 2018 Collaborative Method Performance Study of the Measurement of Nicotine, Its Metabolites, and Total Nicotine Equivalents in Human Urine Wang L, Bernert JT, Benowitz NL, Feng J, Jacob P 3rd, McGahee E, Caudill SP, Scherer G, Scherer M, Pluym N, Doig MV, Newland K, Murphy SE, Caron NJ, Sander LC, Shimizu M, Yamazaki H, Kim S, Langman LJ, Pritchett JS, Sniegoski LT, Li Y, Blount BC, Pirkle JL Cancer Epidemiology, Biomarkers & Prevention, Volume 27, doi: 10.1158/1055-9965 publication March 2014 Saliva as a matrix for human biomonitoring in occupational and environmental medicine Michalke B, Rossbach B, Göen T, Schäferhenrich A, Scherer G International Archives of Occupational and Environmental Health, volume 88, doi: 10.1007/s00420-014-0938-5 publication January 2014 Simple, fast and sensitive LC-MS/MS analysis for the simultaneous quantification of nicotine and 10 of its major metabolites Piller M, Gilch G, Scherer G, Scherer M Journal of Chromatography B, Volumes 951–952, doi: 10.1016/j.jchromb.2014.01.025 publication February 2014 Untargeted metabolomic profiling in saliva of smokers and nonsmokers by a validated GC-TOF-MS method Mueller DC, Piller M, Niessner R, Scherer M, Scherer G Journal of Proteome Research, Volume 13, doi: 10.1021/pr401099r Conference contribution October 2013 Assessing the exposure to ‘smoke’ from electronic cigarettes by biomarkers Scherer G, Hagedorn H-W, Riedel K, Scherer M CORESTA SSPT 2013, Seville, Spain Conference contribution October 2016 Ultra-sensitive method for the determination of nicotine in PK studies with next generation products Wanderer F, Pluym N, Scherer G, Scherer M CORESTA Congress 2016, Berlin, Germany Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–study design Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients – methods Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution February 2018 Biomarkers of exposure specific to e-vapor products based on stable-isotope labeled ingredients Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N SRNT 2018, Baltimore, USA publication March 2022 Assessment of nicotine delivery and uptake in users of various tobacco / nicotine products Scherer G, Mütze J, Pluym N, Scherer M Current Research in Toxicology, Volume 3, 100067 publication September 2022 Urinary cotinine and exposure to passive smoke in children and adolescents in Germany – Human biomonitoring results of the German Environmental Survey 2014–2017 (GerES V) Domenica Hahn, Maria Schmied-Tobies, Enrico Rucic, Nikola Pluym, Max Scherer, Malgorzata Debiak, Aline Murawski, Marike Kolossa-Gehring Environmental Research doi.org/10.1016/j.envres.2022.114320 Conference contribution September 2022 The impact of European legislation on the reduction of Environmental Tobacco Smoke and Polycyclic Aromatic Hydrocarbons between 1995 and 2019 in Germany Therese Burkhardt, Max Scherer, Gerhard Scherer, Till Weber, Nikola Pluym, Marike Kolossa-Gehring ISES 2022, Lisbon publication October 2022 Time trend of exposure to secondhand tobacco smoke and polycyclic aromatic hydrocarbons between 1995 and 2019 in Germany – Showcases for successful European legislation Therese Burkhardt, Max Scherer, Gerhard Scherer, Nikola Pluym, Till Weber, Marike Kolossa-Gehring Environmental Research, doi: 10.1016/j.envres.2022.114638
Creatinine	Normalization of urinary biomarker concentrations	Urine	Photometry
Cyanoethylmercapturic acid (CEMA)	Acrylonitrile	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x publication April 2014 A correlation study applied to biomarkers of internal and effective dose for acrylonitrile and 4-aminobiphenyl in smokers Scherer G, Newland K, Papadopoulou E, Minet E Biomarkers, Volume 19(4), doi: 10.3109/1354750X.2014.910271 Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Cyanoethylvalin (CeVal)	Acrylonitrile	Blood	GC-MS/MS	publication April 2014 A correlation study applied to biomarkers of internal and effective dose for acrylonitrile and 4-aminobiphenyl in smokers Scherer G, Newland K, Papadopoulou E, Minet E Biomarkers, Volume 19(4), doi: 10.3109/1354750X.2014.910271 publication March 2006 Urinary Mercapturic Acids and a Hemoglobin Adduct for the Dosimetry of Acrylamide Exposure in Smokers and Nonsmokers Urban M, Kavvadias D, Riedel K, Scherer G, Tricker AR Inhalation Toxicology, Volume 18, doi: 10.1080/08958370600748430 Conference contribution October 2019 Clinical biomarkers of compliance for use in potentially reduced-risk product switching studies McEwan M, Gale N, Hardie G, Scherer M, Pluym N, Ebajemito JK, Camacho OM, Griffiths A, Proctor CJ CORESTA SSPT 2019, Hamburg, Germany
Dihydroxybutenylmercapturic acid (DHBMA)	1,3-Butadiene	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Dihydroxypropylmercapturic acid (DHPMA)	Glycidol	Urine	LC-MS/MS	Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients – methods Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2018 Biomarkers of exposure specific to e-vapor products based on stable-isotope labeled ingredients Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N SRNT 2018, Baltimore, USA Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA publication June 2021 Assessment of the potential vaping-related exposure to carbonyls and epoxides using stable isotope-labeled precursors in the e-liquid Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Niessner R, Pluym N Archives of Toxicology, doi:10.1007/s00204-021-03097-x publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Ethylmercapturic acid (EMA)	Ethylating agents	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada
Glycidamidemercapturic acid (GAMA)	Acrylamide	Urine	LC-MS/MS	publication October 2020 Benzene metabolite SPMA and acrylamide metabolites AAMA and GAMA in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Schwedler G, Murawski A, Schmied-Tobies MIH, Rucic E, Scherer M, Pluym N, Scherer G, Bethke R, Kolossa-Gehring M Environmental Research, Volume 192, doi: 10.1016/j.envres.2020.110295 publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x publication March 2006 Urinary Mercapturic Acids and a Hemoglobin Adduct for the Dosimetry of Acrylamide Exposure in Smokers and Nonsmokers Urban M, Kavvadias D, Riedel K, Scherer G, Tricker AR Inhalation Toxicology, Volume 18, doi: 10.1080/08958370600748430 Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Hildebrandt acid	Geraniol	Urine	LC-MS/MS	publication January 2022 Time trend of the exposure to geraniol in 24-h urine samples derived from the German Environmental Specimen Bank from 2004 to 2018 Pluym N, Stoeckelhuber M, Weber T, Scherer G, Scherer M, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 239, doi.org/10.1016/j.ijheh.2021.113880
Isoprenemercapturic acid (IPMA-3)	Isoprene	Urine	LC-MS/MS
Lysmerol	Lysmeral (Lilial, Butylphenyl methylpropional)	Urine	LC-MS/MS	publication November 2020 Human biomonitoring in urine samples from the Environmental Specimen Bank reveals a decreasing trend over time in the exposure to the fragrance chemical lysmeral from 2000 to 2018 Scherer M, Petreanu W, Weber T, Scherer G, Pluym N, Kolossa-Gehring M Chemosphere, Volume 265, doi: 10.1016/j.chemosphere.2020.128955 publication August 2020 Metabolites of the fragrance 2-(4-tert-butylbenzyl)propionaldehyde (lysmeral) in urine of children and adolescents in Germany - Human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Fiedler N, Schmied-Tobies MIH, Rucic E, Schwedler G, Stoeckelhuber M, Scherer G, Pluym N, Scherer M, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 229, 10.1016/j.ijheh.2020.113594 publication July 2016 A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the human biomonitoring of non-occupational exposure to the fragrance 2-(4-tert-butylbenzyl)-propionaldehyde (lysmeral) Pluym N, Krnac D, Gilch G, Scherer M, Leibold E, Scherer G Analytical and Bioanalytical Chemistry, Volume 408, doi: 10.1007/s00216-016-9702-x publication September 2016 Human metabolism and excretion kinetics of the fragrance lysmeral after a single oral dosage Scherer M, Koch HM, Schütze A, Pluym N, Krnac D, Gilch G, Leibold E, Scherer G International Journal of Hygiene and Environmental Health, Volume 220, doi: 10.1016/j.ijheh.2016.09.005
MTCA (2-methyl-thiazolidine-4-carboxylic acid)	Acetaldehyde	Plasma, Urine	LC-MS/MS	publication August 2020 A novel quantification method for sulfur-containing biomarkers of formaldehyde and acetaldehyde exposure in human urine and plasma samples Landmesser A, Scherer G, Pluym N, Niessner R, Scherer M Analytical and Bioanalytical Chemistry, volume 412, doi: 10.1007/s00216-020-02888-y Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA
MTCG (2-methyl-thiazolidine-4-carbonyl-glycine)	Acetaldehyde	Plasma, Urine	LC-MS/MS	publication August 2020 A novel quantification method for sulfur-containing biomarkers of formaldehyde and acetaldehyde exposure in human urine and plasma samples Landmesser A, Scherer G, Pluym N, Niessner R, Scherer M Analytical and Bioanalytical Chemistry, volume 412, doi: 10.1007/s00216-020-02888-y Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA
Methylcarbamoylmercapturic acid (AMCC)	N,N-Dimethylformamide	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Methylmercapturic acid (MMA)	Methylating agents	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Monohydroxybutenylmercapturic acid 1 (1-MHBMA)	1,3-Butadiene	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
Monohydroxybutenylmercapturic acid 2 (2-MHBMA)	1,3-Butadiene	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
N-Nitrosonornicotine (NNN)	NNN	Urine, Plasma, Saliva, ETS, Aerosol	LC-MS/MS, GC-MS	publication March 2014 Saliva as a matrix for human biomonitoring in occupational and environmental medicine Michalke B, Rossbach B, Göen T, Schäferhenrich A, Scherer G International Archives of Occupational and Environmental Health, volume 88, doi: 10.1007/s00420-014-0938-5 publication December 2009 Determination of tobacco-specific N-nitrosamines in urine of smokers and non-smokers Kavvadias D, Scherer G, Cheung F, Errington G, Shepperd J, McEwan M Biomarkers, Volume 14, doi: 10.3109/13547500903242883 publication March 2009 Simultaneous determination of four tobacco-specific N-nitrosamines (TSNA) in human urine Kavvadias D, Scherer G, Urban M, Cheung F, Errington G, Shepperd J, McEwan M Journal of Chromatography B, Volume 877, doi: 10.1016/j.jchromb.2009.03.009 Conference contribution October 2019 NNN a suitable biomarker of exposure from smoke and new nicotine delivery products? Pluym N, Taucher J, Scherer G, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution October 2021 Quantitative analysis of NNN in plasma by LC-MS/MS: a suitable alternative to urinary determination to assess the exposure to this important toxicant Pluym N, Sibul F, Jin X, Edmiston J, Sarkar M, Scherer G, Scherer M CORESTA SSPT 2021, Virtual publication March 2022 Assessment of the Exposure to NNN in the Plasma of Smokeless Tobacco Users Pluym N, Scherer G, Edmiston J, Jin X, Sarkar M, Scherer M Chemical Research in Toxicology, doi:10.1021/acs.chemrestox.1c00431 publication March 2022 Assessment of the Exposure to Tobacco-Specific Nitrosamines and Minor Tobacco Alkaloids in Users of Various Tobacco/Nicotine Products Scherer G, Scherer M, Mütze J, Hauke T, Pluym N Chemical Research in Toxicology, doi: 10.1021/acs.chemrestox.2c00020
NNAL	NNK (Nicotine-derived nitrosamine ketone)	Urine	LC-MS/MS	publication January 2018 Review of biomarkers to assess the effects of switching from cigarettes to modified risk tobacco products Peck MJ, Sanders EB, Scherer G, Luedicke F, Weitkunat R Biomarkers, Volume 23(3), doi: 10.1080/1354750X.2017.1419284 publication December 2009 Determination of tobacco-specific N-nitrosamines in urine of smokers and non-smokers Kavvadias D, Scherer G, Cheung F, Errington G, Shepperd J, McEwan M Biomarkers, Volume 14, doi: 10.3109/13547500903242883 publication March 2009 Simultaneous determination of four tobacco-specific N-nitrosamines (TSNA) in human urine Kavvadias D, Scherer G, Urban M, Cheung F, Errington G, Shepperd J, McEwan M Journal of Chromatography B, Volume 877, doi: 10.1016/j.jchromb.2009.03.009 publication March 2022 Assessment of the Exposure to Tobacco-Specific Nitrosamines and Minor Tobacco Alkaloids in Users of Various Tobacco/Nicotine Products Scherer G, Scherer M, Mütze J, Hauke T, Pluym N Chemical Research in Toxicology, doi: 10.1021/acs.chemrestox.2c00020
Propylene glycol (PG)	Propylene glycol	Urine, Plasma, Serum, Saliva, PBS, Aerosol, ETS, e-liquids	LC-MS/MS	publication March 2019 Biomarkers of Exposure Specific to E-vapor Products Based on Stable-Isotope Labeled Ingredients Landmesser A, Scherer M, Pluym N, Sarkar M, Edmiston J, Niessner R, Scherer G Nicotine & Tobacco Research, Volume 21, doi: 10.1093/ntr/nty204 Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria publication October 2021 1,2-Propylene Glycol: A Biomarker of Exposure Specific to e-Cigarette Consumption Burkhardt T, Pluym N, Scherer G, Scherer M Separations, 8, 180, doi: 10.3390/separations8100180 Conference contribution March 2022 Propylene glycol: A Biomarker of Exposure specific to E-Cigarette Consumption Burkhardt T, Pluym N, Scherer G, Scherer M SRNT 2022, Baltimore (Hybrid), USA Conference contribution September 2022 Propylene glycol as a specific biomarker for e-cigarette use Showcase of a novel Orbitrap-MS-based exposomics approach Nikola Pluym, Therese Burkhardt, Alpesh Kachhadia, Gerhard Scherer, Max Scherer ISES 2022, Lisbon
S-Benzylmercapturic acid (SBMA)	Toluene	Urine	LC-MS/MS	publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
S-Phenylmercapturic acid (SPMA)	Benzene	Urine	LC-MS/MS	publication October 2020 Benzene metabolite SPMA and acrylamide metabolites AAMA and GAMA in urine of children and adolescents in Germany - human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Schwedler G, Murawski A, Schmied-Tobies MIH, Rucic E, Scherer M, Pluym N, Scherer G, Bethke R, Kolossa-Gehring M Environmental Research, Volume 192, doi: 10.1016/j.envres.2020.110295 publication May 2015 Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods Pluym N, Gilch G, Scherer G, Scherer M Analytical and Bioanalytical Chemistry, Volume 407, doi: 10.1007/s00216-015-8719-x Conference contribution October 2014 Analysis of 18 Urinary Mercapturic Acids by two Multiplex-LC-MS/MS Methods Pluym N, Gilch G, Scherer G, Scherer M CORESTA Congress 2014, Quebec, Canada publication October 2022 Comparison of urinary mercapturic acid excretions in users of various tobacco/nicotine products Gerhard Scherer, Nikola Pluym, Max Scherer Drug Testing and Analysis DOI: 10.1002/dta.3372
TBBA (tert-Butyl benzoic acid)	Lysmeral (Lilial, Butylphenyl methylpropional)	Urine	LC-MS/MS	publication November 2020 Human biomonitoring in urine samples from the Environmental Specimen Bank reveals a decreasing trend over time in the exposure to the fragrance chemical lysmeral from 2000 to 2018 Scherer M, Petreanu W, Weber T, Scherer G, Pluym N, Kolossa-Gehring M Chemosphere, Volume 265, doi: 10.1016/j.chemosphere.2020.128955 publication August 2020 Metabolites of the fragrance 2-(4-tert-butylbenzyl)propionaldehyde (lysmeral) in urine of children and adolescents in Germany - Human biomonitoring results of the German Environmental Survey 2014-2017 (GerES V) Murawski A, Fiedler N, Schmied-Tobies MIH, Rucic E, Schwedler G, Stoeckelhuber M, Scherer G, Pluym N, Scherer M, Kolossa-Gehring M International Journal of Hygiene and Environmental Health, Volume 229, 10.1016/j.ijheh.2020.113594 publication July 2016 A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the human biomonitoring of non-occupational exposure to the fragrance 2-(4-tert-butylbenzyl)-propionaldehyde (lysmeral) Pluym N, Krnac D, Gilch G, Scherer M, Leibold E, Scherer G Analytical and Bioanalytical Chemistry, Volume 408, doi: 10.1007/s00216-016-9702-x publication September 2016 Human metabolism and excretion kinetics of the fragrance lysmeral after a single oral dosage Scherer M, Koch HM, Schütze A, Pluym N, Krnac D, Gilch G, Leibold E, Scherer G International Journal of Hygiene and Environmental Health, Volume 220, doi: 10.1016/j.ijheh.2016.09.005
Thiazolidine-4-carbonyl-glycine (TCG)	Formaldehyde	Plasma, Urine	LC-MS/MS	publication August 2020 A novel quantification method for sulfur-containing biomarkers of formaldehyde and acetaldehyde exposure in human urine and plasma samples Landmesser A, Scherer G, Pluym N, Niessner R, Scherer M Analytical and Bioanalytical Chemistry, volume 412, doi: 10.1007/s00216-020-02888-y Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA
Thiazolidine-4-carboxylic acid (TCA)	Formaldehyde	Plasma, Urine	LC-MS/MS	publication August 2020 A novel quantification method for sulfur-containing biomarkers of formaldehyde and acetaldehyde exposure in human urine and plasma samples Landmesser A, Scherer G, Pluym N, Niessner R, Scherer M Analytical and Bioanalytical Chemistry, volume 412, doi: 10.1007/s00216-020-02888-y Conference contribution October 2018 Novel biomarkers to characterize exposure to aldehydes from e-vapor products based on stable-isotope constituents Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2018 Analytical methods for the determination of carbonyls in aerosol from e-vapor products and cigarette smoke and their urinary biomarkers of exposure Landmesser A, Scherer M, Scherer G, Pluym N CORESTA Congress 2018, Kunming, China Conference contribution October 2019 Analysis of aldehydes, propylene oxide and glycidol in tobacco smoke and e-cigarette vapor and their respective urinary biomarkers Landmesser A, Scherer G, Pluym N, Scherer M CORESTA SSPT 2019, Hamburg, Germany Conference contribution February 2019 Novel biomarkers to characterize the specific exposure to aldehydes or epoxides from e-vapor products based on stable-isotope labeled constituents Landmesser A, Scherer M, Scherer G, Pluym N SRNT 2019, San Francisco, USA
Thiocyanate (SCN)	Hydrogen cyanide	Plasma, Urine, Serum, Saliva	GC-MS	publication January 2018 Review of biomarkers to assess the effects of switching from cigarettes to modified risk tobacco products Peck MJ, Sanders EB, Scherer G, Luedicke F, Weitkunat R Biomarkers, Volume 23(3), doi: 10.1080/1354750X.2017.1419284 publication March 2014 Saliva as a matrix for human biomonitoring in occupational and environmental medicine Michalke B, Rossbach B, Göen T, Schäferhenrich A, Scherer G International Archives of Occupational and Environmental Health, volume 88, doi: 10.1007/s00420-014-0938-5
ortho-Toluidine	ortho-Toluidine	Urine, ETS	GC-MS	publication April 2006 Determination of three carcinogenic aromatic amines in urine of smokers and nonsmokers Riedel K, Scherer G, Engl J, Hagedorn H-W, Tricker AR Journal of Analytical Toxicology, Volume 30, doi: 10.1093/jat/30.3.187 publication November 2022 Assessment of the Exposure to Aromatic Amines in Users of Various Tobacco/Nicotine Products Gerhard Scherer, Kirsten Riedel, Nikola Pluym, Max Scherer ACS Omega, doi: 10.1021/acsomega.2c06130
trans,trans-muconic acid (tt-MA)	Benzene	Urine	GC-MS
trans-3'-Hydroxycotinine (3-OH-Cot)	Nicotine	Urine, Plasma, Serum, Saliva	LC-MS/MS	publication March 2019 Biomarkers of Exposure Specific to E-vapor Products Based on Stable-Isotope Labeled Ingredients Landmesser A, Scherer M, Pluym N, Sarkar M, Edmiston J, Niessner R, Scherer G Nicotine & Tobacco Research, Volume 21, doi: 10.1093/ntr/nty204 publication May 2018 Collaborative Method Performance Study of the Measurement of Nicotine, Its Metabolites, and Total Nicotine Equivalents in Human Urine Wang L, Bernert JT, Benowitz NL, Feng J, Jacob P 3rd, McGahee E, Caudill SP, Scherer G, Scherer M, Pluym N, Doig MV, Newland K, Murphy SE, Caron NJ, Sander LC, Shimizu M, Yamazaki H, Kim S, Langman LJ, Pritchett JS, Sniegoski LT, Li Y, Blount BC, Pirkle JL Cancer Epidemiology, Biomarkers & Prevention, Volume 27, doi: 10.1158/1055-9965 publication March 2014 Saliva as a matrix for human biomonitoring in occupational and environmental medicine Michalke B, Rossbach B, Göen T, Schäferhenrich A, Scherer G International Archives of Occupational and Environmental Health, volume 88, doi: 10.1007/s00420-014-0938-5 publication January 2014 Simple, fast and sensitive LC-MS/MS analysis for the simultaneous quantification of nicotine and 10 of its major metabolites Piller M, Gilch G, Scherer G, Scherer M Journal of Chromatography B, Volumes 951–952, doi: 10.1016/j.jchromb.2014.01.025 publication February 2014 Untargeted metabolomic profiling in saliva of smokers and nonsmokers by a validated GC-TOF-MS method Mueller DC, Piller M, Niessner R, Scherer M, Scherer G Journal of Proteome Research, Volume 13, doi: 10.1021/pr401099r Conference contribution October 2013 Assessing the exposure to ‘smoke’ from electronic cigarettes by biomarkers Scherer G, Hagedorn H-W, Riedel K, Scherer M CORESTA SSPT 2013, Seville, Spain Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–study design Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients–results Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution October 2017 Biomarkers of exposure specific to e-vapor products based on stable-isotope labelled ingredients – methods Landmesser A, Pluym N, Scherer G, Sarkar M, Edmiston J, Scherer M CORESTA SSPT 2017, Kitzbühel, Austria Conference contribution February 2018 Biomarkers of exposure specific to e-vapor products based on stable-isotope labeled ingredients Landmesser A, Scherer M, Scherer G, Sarkar M, Edmiston J, Pluym N SRNT 2018, Baltimore, USA publication March 2022 Assessment of nicotine delivery and uptake in users of various tobacco / nicotine products Scherer G, Mütze J, Pluym N, Scherer M Current Research in Toxicology, Volume 3, 100067

Pioneers in biomarker research

Almost all of the biomarker methods developed at ABF were published in peer-reviewed journals, a fact that supports the validity, transparency and scientific evidence of the bioanalysis especially of importance for regulatory submissions